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Rocky Mountain Diagnostics, Inc.

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Buying Guide for Catecholamines

Catecholamine Assays Research Applications

Sample / KitAvailable Sample VolumeRecommended AssayAvailable Assay TypeAlternative Assays (Increased Sensitivity)
Urine 25 µl» Series BA 7000 RIA/ELISA» Series BA 6000 or BA 5000
10 µl» Series BA 6000 RIA/ELISA» Series BA 5000
< 10 µl» Series BA 5000 ELISA» Contact us
Plasma 600 µl» Series BA 4000 ELISA» Series BA 5000
300 µl» Series BA 6000 RIA/ELISA» Series BA 5000
< 300 µl or < 600µl» Series BA 5000 ELISA» Contact us
Other 1-750 µl» Series BA 5000 ELISA» As the sensitivity depends on the sample volume a detailed knowledge of the applicable sample volume is advisable. Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. and our specialists will assist you to implement the best protocol.
Kit / SampleSample VolumeMeasuring Range EpinephrineAnalytical Sensitivity EpinephrineMeasuring Range NorepinephrineAnalytical Sensitivity NorepinephrineMeasuring Range DopamineAnalytical Sensitivity Dopamine
BA R-7000 URINE 25 µl 1.5 - 240 ng /ml 0.5 ng /ml 7.5 - 1200 ng /ml 1.7 ng /ml 25 - 4000 ng /ml 3 ng /ml
BA E-7000 URINE 25 µl 1.5 - 240 ng /ml 0.33 ng /ml 7.5 - 1200 ng /ml 1.33 ng /ml 25 - 4000 ng /ml 0.83 ng /ml
BA E-4000 PLASMA 600 µl 16.7 - 3333 pg /ml 5 pg /ml 83.3 - 16667 pg /ml 25 pg /ml 30 - 13333 pg /ml 10 pg /ml
BA R-6000 URINE 10 µl 1 - 200 ng /ml 0.3 ng /ml 5 - 1000 ng /ml 1.5 ng /ml 10 - 2000 ng /ml 4.5 ng /ml
BA R-6000 PLASMA 300 µl 33 - 6667 pg /ml 10 pg /ml 167 - 33333 pg /ml 50 pg /ml 60 - 26667 pg /ml 20 pg /ml
BA E-6000 URINE 10 µl 1 - 200 ng /ml 0.3 ng /ml 5 - 1000 ng /ml 1.5 ng /ml 10 - 2000 ng /ml 4.5 ng /ml
BA E-6000 PLASMA 300 µl 33 - 6667 pg /ml 10 pg /ml 167 - 33333 pg /ml 50 pg /ml 75 - 33333 pg /ml 25 pg /ml
BA E-5100-5300 (1-750µl) e.g. 10 µl 0.45 - 45 ng /ml 0.3 ng /ml* 0.45 - 45 ng /ml 0.2 ng /ml* 0.9 - 90 ng /ml 0.7 ng /ml*
e.g. 100 µl 45 - 4500 pg /ml 30 pg /ml* 45 - 450 pg /ml 20 pg /ml* 90 - 900 pg /ml 70 ng /ml*
e.g. 750 µl 6 - 600 pg /ml 4 pg /ml* 6 - 600 pg /ml 2.7 pg /ml* 12 - 1200 pg /ml 9.3 pg /ml*
BA E-5400-5500 (1-750µl) e.g. 10 µl 0.9 - 90 ng /ml 0.6 ng /ml* 0.9 - 90 ng /ml 0.4 ng /ml* 1.8 - 180 ng /ml 1.4 ng /ml*
e.g. 100 µl 90 - 9000 pg /ml 60 pg /ml* 90 - 9000 pg /ml 40 pg /ml* 180 - 1800 pg /ml 140 pg /ml*
e.g. 750 µl 12 - 1200 pg /ml 8 pg /ml* 12 - 1200 pg /ml 5.3 pg /ml* 24 - 2400 pg /ml 18.7 pg /ml*
BA E-5600 (1-750µl) e.g. 10 µl 1.35 - 135 ng /ml 0.9 ng /ml* 1.35 - 135 ng /ml 0.6 ng /ml* 2.7 - 270 ng /ml 2.1 ng /ml*
e.g. 100 µl 135 - 13500 pg /ml 90 pg /ml* 135 - 13500 pg /ml 60 pg /ml* 270 - 27000 pg /ml 210 pg /ml*
e.g. 750 µl 18 - 1800 pg /ml 12 pg /ml* 18 - 1800 pg /ml 8 pg /ml* 36 - 3600 pg /ml 28 pg /ml*

Catecholamine Assays for Clinical Use

SampleAvailable Sample VolumeRecommended AssayAvailable Assay TypeAssay Total Time
Urine 25 µl» Series BA 7000 RIA/ELISA» RIA 2.5 h / ELISA 2 h
Plasma 10 µl» Series BA 6000 RIA/ELISA» ELISA 5 h
Urine and/or Plasma < 10 µl» Series BA 5000 RIA/ELISA» RIA 4 h or O.N. / ELISA 5 h
Kit / SampleSample VolumeMeasuring Range EpinephrineAnalytical Sensitivity EpinephrineMeasuring Range NorepinephrineAnalytical Sensitivity NorepinephrineMeasuring Range DopamineAnalytical Sensitivity Dopamine
BA R-7000 URINE 25 µl 1.5 - 240 ng /ml 0.5 ng /ml 7.5 - 1200 ng /ml 1.7 ng /ml 25 - 4000 ng /ml 3 ng /ml
BA E-7000 URINE 25 µl 1.5 - 240 ng /ml 0.33 ng /ml 7.5 - 1200 ng /ml 1.33 ng /ml 25 - 4000 ng /ml 0.83 ng /ml
BA E-4000 PLASMA 600 µl 16.7 - 3333 pg /ml 5 pg /ml 83.3 - 16667 pg /ml 25 pg /ml 30 - 13333 pg /ml 10 pg /ml
BA R-6000 URINE 10 µl 1 - 200 ng /ml 0.3 ng /ml 5 - 1000 ng /ml 1.5 ng /ml 10 - 2000 ng /ml 4.5 ng /ml
BA R-6000 PLASMA 300 µl 33 - 6667 pg /ml 10 pg /ml 167 - 33333 pg /ml 50 pg /ml 60 - 26667 pg /ml 20 pg /ml
BA E-6000 URINE 10 µl 1 - 200 ng /ml 0.3 ng /ml 5 - 1000 ng /ml 1.5 ng /ml 10 - 2000 ng /ml 4.5 ng /ml
BA E-6000 PLASMA 300 µl 33 - 6667 pg /ml 10 pg /ml 167 - 33333 pg /ml 50 pg /ml 75 - 33333 pg /ml 25 pg /ml

HistaSure Dipstick


HistaSure - Dipstick

The worlds first production dip-stick for the detection of Histamine in Fish is here! In Just minutes you can screen for the presence of histamine. We pre-set the cut to international standards of 50ppm, but the system is easily changed to allow for adjustable cut-offs in order to meet your QC needs.

Instructions:


  1. Mix 10 grams of tuna with 240 mL of water
  2. Add 100 µl of the sample to the tube containing 500 ul of Acylation Buffer - Vortex to mix
  3. Add 100 µl of the acylation reagent to the tube in step 2
  4. Vortex to mix and incubate for 5 minutes
  5. Pipette 100 ul of the acylated sample to the Sample Diluent.
  6. Vortex to mix Add 100 ul to the cassette
  7. Read results after 5 minutes.

Advantages

  • Fast results in minutes
  • No special equipment needed
  • Easy interpretation
  • Can be performed Dockside
  • Reuse the sample in our ELISA

Results and Interpretation:

The visual inspection of the LF-cassettes can give the following results: 2 lines or 1 line.

2 lines:

The histamine concentration of the sample is below 50 ppm; the sample has passed. Example: 2 lines are visible - the sample has passed2line-histasure

1 line:

Only the control line is visible. The histamine concentration of the sample is above 50 ppm; the sample has failed and further investigations have to be performed. Example: 1 line is visible – the sample has failed.1line-histasure
 

Dopamine, ELISA kits

Use our Dopamine ELISA with any biological fluid!



Looking for an efficient method to test Dopamine in plasma, urine, cell cultures or any other biological fluid? Are you testing mice, rats, cows, sheep, pigs, horses, flora,  platypuses or should we say platypodes? Then you have come to the right place!

We offer several types of Dopamine ELISA kits:

BA E-5300 - Research Dopamine ELISA For research use on any biological fluid

BA E-6300 Fast Track ELISA kit for Dopamine in Plasma or Urine  - This kit is best used for human samples. 

BA E-7300 Fast track ELISA kit for Urine - This kit is also best used with a human sample, but is faster than the BA E-6300.

Plus we offer all of the above in combination kit so if you need Dopamine and Epinephrine, we have you covered, and if you need dopamine, with epinephrine, and norepinephrine, we have kits for all three as well!

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Serotonin ELISA / EIA

Serotonin ELISA Kits

About Serotonin:

Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain.Central-serotonergic neurones influence
physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoma, endogenous depression and schizophrenia.

Our Serotonin ELISA

The assay kit provides materials for the quantitative determination of derivatised serotonin in serum, plasma, urine, platelets, tissue homogenates, cell culture supernatants, CSF, and other biological fluids. The derivatisation is part of the preparation of the samples. By using the acylation reagent the serotonin is quantitatively derivatised into N-acylserotonin. The competitive Serotonin EIA kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB/peroxidase reaction is read at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.

 

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The HistaSure™ ELISA Fast Track

Histamine Analysis

Screening, Quantitative, and Semi-Quantitative

Whether its fresh off the boat, or further processed, RMD offers a number of solutions to Histamine testing, that are built on many years of experience in clinical diagnostics. Quality is the cornerstone of any business, and we are here to help you reinforce that commitment to quality with cutting-edge technologies.
AOAC

 

Download the Instructions

HistaSure - Fully Quantative

New and improved ELISA method that offers fully quantitative results in 30 minutes! With a simple water extraction and our improved ELISA method, we have surpassed similar methods in speed and usability. Ideal for high volume testing, or small runs, this new ELISA is a breakthrough.
The HistaSure™ ELISA Fast Track has been validated and certified as a Performance Tested MethodSM by the AOAC Research Institute as an effective method for the quantitative determination of histamine in fish. The ease of use and wide-ranging application areas make this assay a versatile and reliable tool for laboratories engaged in food safety. This assay is an important piece of any seafood processor's control strategy in their HACCP program to address the hazard of scombrotoxin formation.

 

Advantages

  • Designed for quantitative determinations (6 controls) or semi-quantitative determinations (1 out of 7 cut-off controls).
  • Run 42 samples in one run quantitatively, or as many as 47 samples in one run semi-quantitatively
  • Quantitative determinations have a high dynamic range between 0 und 300 ppm
  • For Semi-Quantitative Determinations: Choose from seven different cut-off points between 3 und 300 ppm
  • Validated and certified by the AOAC Research Institute for many different fish species and matrices such as fresh/frozen yellowfin tuna, canned tuna-chunk light in water, fresh/frozen mahimahi, canned sardines in oil and fish meal. No expensive or complicated equipment required Long shelf-life greater than 12 Months
  • On a regular basis the assay is successfully used in the external quality assessment schemes of FAPAS

Norepinephrine ELISA Kits

Norepinephrine ELISA kit for plasma and urine:

The BA E-6000, BA E-4000 and Ba E-7000 series of kits are designed primarily for testing human samples, although like the research kit it can be used on any biological fluid. Sometimes if the sample is other than human but your expected values from your sample fit perfectly to the curve, and you have enough sample to work with, we will recommend this more economic version.

Norepinephrine ELISA kit - Research:

The BA E-5200 kit is designed primiarily for testing samples that vary not only in origin but also size. It can be used on any biological fluid. For example, if you have a sample from a Rat, be it brain homogenate, urine, plasma, or even bone, you can use this flexible solution to test your sample effectively. Thanks in part to the innovative extraction step, we make all samples equal, to put it in simple terms. 

Can it really be used with any biological fluid? 

    YES! and we've got the publications to back that up. Here is a short list of publications that pertain specifically  to our catecholamine kits. Note: the 2-CAT and 3-CAT ELISA, as well as our RIA kits are included here as well because they all function according to the same principles, only the detection method differs.

 

Browse and Shop our Products

 

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Epinephrine ELISA Kits

Epinephrine ELISA kit for Urine and Plasma:

The BA E-4000, BA E-6000, and BA E-7000 series kits are designed primarily for testing human samples, although in many cases excepting the 7000's, they too can be used on any biological fluid. Sometimes if the sample is other than human but your expected values from your sample fit perfectly to the curve, and you have enough sample to work with, we will recommend this more economic version.

 

Epinephrine ELISA kit for Research - BA E-5100:

The BA E-5100 kit is designed primarily for testing samples that vary not only in origin but also size. It can be used on any biological fluid. For example, if you have a sample from a Rat, be it brain homogenate, urine, plasma, or even bone, you can use this flexible solution to test your sample effectively. Thanks to an innovative extraction step, we make all samples equal, to put it in simple terms. 

 

Can it really be used with any biological fluid? 

    YES! and we've got the publications to back that up as well. Here is a short list of publications that pertain specifically  to our catecholamine kits. Note: the 2-CAT and 3-CAT ELISA, as well as our RIA kits are included here as well because they all function according to the same principles, only the detection method differs.

 

Browse and Shop our Products

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Cross Reactivities of Biogenic Amine Kits

Below is a chart that identifies which parameters we have tested using our own kits. Note that we do not specify which kit, because as changes are made to the kits over time, the basic principles continue to hold true for all our kits.

Important: each laboratory should evaluate the protocol according to official guidelines (e.g. GLP, etc.).

The following contains a chart with the documented cross-reactivities of our kits. Although we are permanentely updating this chart we would kindly ask you to contact our product specialists to discuss your special project.

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Serotonin Assays -From The Serotonin Experts

The most comprehensive and innovative serotonin panel available- only from LDN

The evolution of our serotonin kits continue their path to better meet the needs of our customers. Whatever sample you're targeting, we have the optimal solution:

Our unique CE-labeled RIA (BA R-8900) and innovative ELISA (BA E-8900) test kits may be used to determine Serotonin in serum, platelet-rich plasma (PRP), platelet-poor plasma (PPP), CSF, urine, cell cultures and tissue homogenates.

Our exclusive Serotonin Research RIA™ (BA E-8900) and Serotonin Research ELISA™ (BA-E-5900) test kits are of highest sensitivity, and therefore, the ideal tool for scientists breaking new grounds.

LDN as a team has decades of experience in the arena of serotonin testing. If you are looking for a customized solution to your testing needs, then you rely on us as an accomplished partner.

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Urinary Metanephrines

Background Information
Metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are physiological breakdown products of the catecholamines adrenaline and noradrenaline, which play an important role as hormones and neurotransmitters. Metanephrine and normetanephrine are excreted in urine or broken down further in the body to the end product of catecholamine metabolism vanilylmandelic acid.

 

The diseases which are associated with an increased concentration of metanephrine and normetanephrine are catecholamine-producing tumors, for example, neuroblastoma, ganglioneuroma, and phaeochromocytoma. Of these, phaeochromocytoma has the greatest significance. Phaeochromocytomas are excessively catecholamine-secreting tumors, which originate in the adrenal medula and can, in combination with operative procedures and anaesthesia, lead to life-threatening situations. Around 10% of all phaeochromocytomas show malignant growth. In most cases, however, they can be cured by operative therapy combined with medication therapy using ?-adrenergen antagonists. An unidentified phaeochromocytoma, on the other hand, poses a serious risk to the patient.

 

The determination of metanephrine and normetanephrine (or catecholamine) concentrations is therefore essential in patients with suspected phaeochromocytoma. These tests are also frequently used in the initial diagnosis of hypertonia.

 

RIA- The assay kit provides materials for the quantitative measurement of normetanephrine and metanephrine in urine. During the sample preparation normetanephrine and metanephrine are quantitatively acylated to their corresponding N-acyl-derivates. The assay procedure follows the basic principle of radioimmunoassays, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labeled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.

 

ELISA-  The competitive EIA kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. The acylated metanephrine and normetanephrine from the sample and solid phase bound nephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase metanephrine and normetanephrine is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate.  The reaction is monitored at 450 nm with the amount of antibody bound to the solid phase nephrine being inversely proportional to the nephrine concentration of the sample.

 

ELISA vs. HPLC Comparison- Urine

The metanephrine and the normetanephrine concentrations in 27 urine samples were assessed using both the LDN EIA (x) and the HPLC method (y) (external QC samples from UK NEQAS). The UK control values are the mean of about 40 different HPLC users, and contain always one pathological sample per sending. The results of linear regression analysis showed the following correlation characteristics: y=0.88x - 0.10, r=0.99 for metanephrine and y=1.09x + 0.3, r=0.99 for normetanephrine.

Bottom Line: The LDN ELISA is comparable to the HPLC method

 
RIA vs. HPLC   Comparison- Urine

The metanephrine and the normetanephrine concentrations in 21 urine samples were assessed using both the LDN RIA (x) and the HPLC method (y) (external QC samples from UK NEQAS). Therefore the correlation between RIA and HPLC is excellent.   UK control values are the mean of about 40 different HPLC users, and contain always one pathological sample per sending. The results of linear regression analysis showed the following correlation characteristics for metanephrine: y=1.01x – 0.09, r=0.99, and for normetanephrine y=0.95x + 0.17, r=0.99

Bottom Line: The LDN RIA is comparable to the HPLC method

 

Advantages of LDN Metanephrine Kits
  • Customer Service! - We use the kits we sell, and we always offer a 24 hour turn around on almost all technical questions.
  • Freedom from drug Interference!
  • Complicated HPLC and LC/MS procedures can now be overcome with easy to use ELISA’s and RIA’s
  • Excellent correlation with other methodologies.
  • QUALITY- Produced according to ISO:13485

Catecholamine ELISA and RIA Immunoassays

Introduction

Adrenaline (epinephrine) and noradrenaline (norepinephrine) together with dopamine constitute the group of catecholamines, which play an important role as hormones and neurotransmitters. Catecholamines are biogenic amines which are synthesized from the amino acid tyrosine via the intermediate product dopa.

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