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Rocky Mountain Diagnostics, Inc.

Dopamine, ELISA kits

Use our Dopamine ELISA with any biological fluid!



Looking for an efficient method to test Dopamine in plasma, urine, cell cultures or any other biological fluid? Are you testing mice, rats, cows, sheep, pigs, horses, flora,  platypuses or should we say platypodes? Then you have come to the right place!

We offer several types of Dopamine ELISA kits:

BA E-5300 - Research Dopamine ELISA For research use on any biological fluid

BA E-6300 Fast Track ELISA kit for Dopamine in Plasma or Urine  - This kit is best used for human samples. 

BA E-7300 Fast track ELISA kit for Urine - This kit is also best used with a human sample, but is faster than the BA E-6300.

Plus we offer all of the above in combination kit so if you need Dopamine and Epinephrine, we have you covered, and if you need dopamine, with epinephrine, and norepinephrine, we have kits for all three as well!

 

Our Method

Here is a little insight as to how we can test any biological fluid, and what makes our system unique and robust:

Noradrenaline (Noradrenaline), Epinephrine (adrenaline), and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyldopamine and then converted enzymatically during the detection procedure into N-acylnormetanephrine, N-acylmetanephrine, and N-acyl-3-methoxy-tyramine. For the ELISA, N-acylnormetanephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine, respectively, are bound to the surface of  microtiter plates. Acylated metanephrines from the sample and solid phase bound N-acylmetanephrines compete for a fixed number of specific rabbit antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the respective solid phase N-acylmetanephrine is detected by a mouse anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The RIA is a conventional double antibody test using the same rabbit antisera.

 

The extraction

 

1. Immobilization

catechol-extraction

2. First Clean-Up

 
catechol-extraction-decant

3. N-Acylation

catechol-extraction-acylate
1. Catecholamines are covalently bound to immobilized m-phenyl boronic acid.
For this step the 3,4 dihydroxy- structure of the catecholamines is mandatory!
2. Remove non-bound biomolecules 3. The immobilized catecholamines are derivatized by use of an activated ester.
For this step the primary or secondary amino groups are mandatory

 

After these essential steps, all other problematic structures are literally washed away, thus removing any interference from similar structures. The subsequent enzymatic conversion to N-acylnormetanephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine, further disguises the molecule, and makes the molecule recognizable to our antibodies.

Our Publications for Dopamine ELISA and RIA

If you needed any further convincing all of these publications are relevant. Some were used with different formats of the same kits. However, all of our catecholamine kits function after the same principals.  We alo sell these kits under a nubmer of name brands, we have included those in our list as well, but rest assured LDN's technology is behind all of those names.

RIA / ELISA

Analyte / Kit

Year

Author

Title

Journal

ELISA 3 CAT 2005 Aviles et al. Effects of Space Flight Conditions on the Function of the Immune System and Catecholamine Production Simulated in a Rodent Model of Hindlimb Unloading NeuroImmunoModulation
ELISA 3 CAT 2006 Kobori et al. Enhanced Catecholamine Synthesis in the Prefrontal Cortex after Traumatic Brain Injury: Implications for Prefrontal Dysfunction Journal of Neurotrauma
ELISA 3 CAT 2006 Khanam et al. Effect of Chromium Picolinate on Modified Forced Swimming Test in Diabetic Rats: Involvement of Serotonergic Pathways and Potassium Channels Basic & Clinical Pharmacology & Toxicology
ELISA 3 CAT 2007 Butte et al. Body Size, Body Composition, and Metabolic Profile Explain Higher Energy Expenditure in Overweight Children Journal of Nutrition: Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions
ELISA 3 CAT 2008 Nogami et al. Liposome-Encapsulated Hemoglobin Transfusion Rescues Rats Undergoing Progressive Hemodilution From Lethal Organ Hypoxia Without Scavenging Nitric Oxide Annals of Surgery
ELISA 3 CAT 2008 O'Donnel et al. Effects of exposure of mice to hindlimb unloading on leukocyte subsets and sympathetic nervous system activity Stress
ELISA 3 CAT 2009 Mobine et al. Encapsulated Pheochromocytoma Cells Secrete Potent Noncatecholamine Factors Tissue engineering Part A
ELISA 3 CAT 2009 Ramiro-Fuentes et al. Morphophysiology of the Zuckerkandl's paraganglion: Effects of dexamethasone and aging Neurobiology of Aging
ELISA 3 CAT 2009 Mobine et al. Pheochromocytoma-Induced Cardiomyopathy is Modulated by the Synergistic Effects of Cell-Secreted Factors Circulation: Heart Failure
ELISA 3 CAT 2009 Snow et al. Catecholamine alterations in pediatric obstructive sleep apnea: Effect of obesity Pediatric Pulmonology
ELISA 3 CAT 2010 Bass et al. Effects of sex and short-term magnesium supplementation on stress responses and longissimus muscle quality characteristics of crossbred cattle J Anim Sci
ELISA 3 CAT 2009 Mobine et al. Ecapsulated Pheochromocytoma Cells Secrete Potent Noncatecholamine Factors Tissue engineering Part A
RIA 3 CAT 2002 Dettling et al. Repeated parental deprivation in the infant common marmoset (callithrix Yescchus, primates) and analysis of its effects on early development Biological Psychiatry
RIA 3 CAT 2006 Mahapatra et al. The chromogranin A fragment catestatin: specificity, potency and mechanism to inhibit exocytotic secretion of multiple catecholamine storage vesicle co-transmitters Journal of Hypertension
RIA 3 CAT 2008 Bates et al. Adaptation to Mild, Intermittent Stress Delays Development of Hyperglycemia in the ZDF Rat Independent of Food Intake: Role of Habituation of the HPA Axis Endocrinology
RIA 3 CAT 2009 Seifert et al. Cerebral oxygenation and metabolism during exercise following three months of endurance training in healthy overweight males Am J Physiol Regul Integr Comp Physiol
EIA Dopamine 2009 Liu et al. Reduction of Dopamine Level Enhances the Attractiveness of Male Drosophila to Other Males PLoS
ELISA Dopamine 2005 Cha et al. Parkin negatively regulates JNK pathway in the dopaminergic neurons of Drosophila PNAS
ELISA Dopamine 2005 Rowell et al. Chemoprevention of Breast Cancer, Proteomic Discovery of Gistein Action in the Rat Mammary Gland International Conference on Diet, Nutrition and Cancer
ELISA Dopamine 2006 Park et al. Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin Nature
ELISA Dopamine 2006 Armentero et al. Peripheral inflammation and neuroprotection: Systemic pretreatment with complete Freund's adjuvant reduces 6-hydroxydopamine toxicity in a rodent model of Parkinson's disease Neurobiology of Disease
ELISA Dopamine 2007 Traska et al. Specification of a Dopaminergic Phenotype from Adult Human Mesenchymal Stem Cells Stem Cells
ELISA Dopamine 2007 Khedr et al. Dopamine Levels After Repetitive Transcranial Magnetic Stimulation of Motor Cortex in Patients with Parkinson´s Disease: Preliminary Results Movement Disorders
ELISA Dopamine 2007 Sontag et al. Repetitive transcranial magnetic stimulation does not influence immunological HL-60 cells and neuronal PC12 cells International Journal of Radiation Biology
ELISA Dopamine 2007 Yang et al. Reversible Unilateral Nigrostriatal Pathway Inhibition Induced Through Expression of Adenovirus-mediated Clostridial Light Chain Gene in the Substantia Nigra NeuroMolecular Medicine
ELISA Dopamine 2008 Sitaraman et al. Serotonin is necessary for place memory in Drosophila PNAS
ELISA Dopamine 2008 Galan-Rodriguez et al. Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts Neurobiology of Disease
ELISA Dopamine 2008 Hamann et al. 9-Methyl-β-carboline up-regulates the appearance of differentiated dopaminergic neurones in primary mesencephalic culture Neurochemistry International
ELISA Dopamine 2008 Matsumoto et al. Attenuation of methamphetamine-induced effects through the antagonism of sigma (s) receptors: Evidence from in vivo and in vitro studies European Neuropsychopharmacology
ELISA Dopamine 2008 Abbiss et al. Effect of carbohydrate ingestion and ambient temperature on muscle fatigue development in endurance-trained male cyclists Journal of Applied Physiology
ELISA Dopamine 2009 Lee et al. Nicotinamide reduces dopamine in postnatal hypothalamus and causes dopamine-deficient phenotype Neuroscience Letters
ELISA Dopamine 2010 Seddik et al. Dried Leaf Extract Protects against Lead-Induced Neurotoxicity in Wistar Rats Europ J Scientific Res
ELISA Dopamine 2009 Kilbourn et al. In vivo [11C]dihydrotetrabenazine binding in rat striatum: sensitivity to dopamine concentrations Nuclear Medicine and Biology
ELISA Dopamine 2009 Panjala et al. Effects of insulin-like growth factor-1 (IGF-1) receptor signaling on rates of apoptosis in retina of dopamine beta hydroxylase (Dbh−/−) knockout mice Autonomic Neuroscience
RIA Dopamine 1999 Watts et al. Short- and long-term heterologous sensitization of adenylate cyclase by D4 dopamine receptors Psychopharmacology
RIA Dopamine 2002 Tran et al. Effect of High Dietary Manganese Intake of Neonatal Rats on Tissue Mineral Accumulation, Striatal Dopamine Levels, and Neurodevelopmental Status Neurotoxicology
RIA Dopamine 2003 Nybo et al. Neurohumoral responses during prolonged exercise in humans Journal of Applied Physiology
RIA Dopamine 2004 Ye et al. Long-term but not short-term blockade of dopamine release in Drosophila impairs orientation during flight in a visual attention paradigm Eur J Neuroscience
RIA Dopamine 2005 Aleander et al. Noradrenergic and dopaminergic effects of (+)-amphetamine-like stimulants in the baboon Papio anubis Synapse
RIA Dopamine 2007 Lee et al. Strain-specific steroidal control of pituitary function Journal of Endocrinology
RIA Dopamine 2008 Haritou et al. Seasonal Changes in Circadian Peripheral Plasma Concentrations of Melatonin, Serotonin, Dopamine and Cortisol in Aged Horses with Cushing’s Disease under Natural Photoperiod Journal of Neuroendocrinology
RIA Dopamine 2008 Liu et al. Increased Dopamine Level Enhances Male–Male Courtship in Drosophila The Journal of Neuroscience
RIA Dopamine 2008 Bork et al. N-Propanoylmannosamine Interferes With O-GlcNAc Modification of the Tyrosine 3-Monooxygenase and Stimulates Dopamine Secretion Journal of neuroscience research   
RIA Dopamine 2009 Xiao Neural cell adhesion molecule NCAM modulates dopamine-related behavior by regulating dopamine D2 receptor internalization in mice (Mus musculus Linnaeus, 1758) Dissertation
ELISA Dopamine Research 2007 Briffa et al. Monoamines and decision making during contests in the hermit crab Pagurus bernhardus Animal Behaviour
ELISA Dopamine Research 2008 Liu et al. Relationship between methamphetamine exposure and matrix metalloproteinase 9 expression Neuroreport
RIA Dopamine Research 2010 Cui et al. Embryonic stem cell-derived L1 overexpressing neural aggregates enhance recovery in Parkinsonian mice Brain
ELISA Dopamine Research 2007 Jaubert et al. Tyrosine Hydroxylase and Dopamine Transporter Expression in Lactotrophs from Postlactating Rats: Involvement in Dopamine-Induced Apoptosis Endocrinology
ELISA dopamine/noradrenaline 2009 Brown et al. Hypoxia inducible factor (HIF)-2a is required for the development of the catecholaminergic phenotype of sympathoadrenal cells Journal of Neurochemistry
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