Call Us Toll Free: 1-877-477-0039

Call Us Toll Free: 1-877-477-0039

Rocky Mountain Diagnostics, Inc.

Serotonin ELISAs

Serotonin ELISA / EIA

Serotonin ELISA Kits

About Serotonin:

Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain.Central-serotonergic neurones influence
physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoma, endogenous depression and schizophrenia.

Our Serotonin ELISA

The assay kit provides materials for the quantitative determination of derivatised serotonin in serum, plasma, urine, platelets, tissue homogenates, cell culture supernatants, CSF, and other biological fluids. The derivatisation is part of the preparation of the samples. By using the acylation reagent the serotonin is quantitatively derivatised into N-acylserotonin. The competitive Serotonin EIA kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB/peroxidase reaction is read at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.

 


Why do we Acylate?

The simple answer is because it makes for a much better assay. The results are certainly worth the 15 minutes it takes to perform this step. By disguising the molecule, we literally wash away all problems in the beginning, so that the end result is a signal that is free from unwanted cross reactivities and interference, as demonstrated by the following charts:

Analytical Specificity
(Cross Reactivity)

Substance

Cross Reactivity (%)

Serotonin

Serotonin

100

Tryptamine

0.19

Melatonin

0.03

5-Hydroxyindole acetic acid

<0.002

Phenylalanine

<0.002

Histidine

<0.002

Tyramine

<0.002

5-Hydroxytryptophan

<0.002


Precision

Intra-Assay

Inter-Assay

 

Sample

Range (ng/mL)

CV (%)

 

Sample

Range (ng/mL)

CV (%)

Serotonin

Urine

1

111 ± 7.3

6.6

Serotonin

Urine

1

84 ± 6.2

7.4

2

355 ± 23

6.4

2

310 ± 25

7.9

Serotonin

Serum

1

110 ± 6.0

5.4

Serotonin

Serum

1

121 ± 7.4

6.0

2

297 ± 12

3.9

2

333 ± 205

6.0

 

A Well referenced and versitile ELISA kit

 

Year

Author

Article Title

Journal/Publication

Species

Specimin

2007 Zhang et al. Bcl-2 family proteins are essential for platelet survival Cell Death and Differentiation dog platelets
2009 Scheideler Einfluss von kurz-, mittel- und langfristiger Belastung auf physiologische Parameter bei Arbeitshunden Dissertation dog  
2006 Yuan et al. A sleep-promoting role for the Drosophila serotonin receptor 1A. Curr Biol drosophila head homogenate
2006 Yuan et al. Serotonin Modulates Circadian Entrainment in Drosophila Neuron drosophila head homogenate
2008 Kidd et al. Luminal regulation of normal and neoplastic human EC cell serotonin release is mediated by bile salts, amines, tastants, and olfactants Am J Physiol Gastrointest Liver Physiol EC cells release
2009 Morando et al. Fluoxetine treatment affects nitrogen waste excretion and osmoregulation in a marine teleost fish Aquatic Toxicology Gulf toadfish plasma
2005 Salloum et al. Release and Elimination of Soluble Vasoactive Factors during Percutaneous Coronary Intervention of Saphenous Vein Grafts: Analysis Using the PercuSurge GuardWire® Distal Protection Device The Journal of invasive Cardiology human serum
2006 Ko et al. Elevated levels of imflammatory and vasoactive Factors in the culprit coronary artey of acute myocardial infarction Tissue engineering and regenerative medicine human serum
2006 Konca et al. The effect of cromoglycate on time-dependent histamine and serotonin concentrations in stored blood products Transfusion and apheresis science human serum
2006 Ko et al. Inflammatory and vasoactive factors in the aspirate from the culprit coronary artery of patients with acute myocardial infarction International Journal of Cardiology human serum
2006 Hou et al. CSF serotonin, 5-hydroxyindolacetic acid and neuropeptide Y levels in severe major depressive disorder Brain Res human CSF
2006   The Study of Quigang Training on Healthy Factors Related to Physiopsychology in Patients with Depression   human plasma
2007 Demirtunc et al. Effects of Doxazosin and Amlodipine on Mean Platelet Volume and Serum Serotonin Level in Patients with Metabolic Syndrome Clinical Drug Investigation human serum
2007 Oben et al. The effect of Cissus quadrangularis (CQR-300) and a Cissus formulation (CORE) on obesity and obesity-induced oxidative stress Lipids Health Disease human heparine plasma
2007 Kälvegren The Role of Chlamydia pneumoniae-induced Platelet Activation in Cardiovascular Disease   human plasma
2007 Kälvegren The Role of Chlamydia pneumoniae-induced Platelet Activation in Cardiovascular Disease Dissertation - Linkoping University Sweden human EDTA plasma
2008 Kälvegren et al. Correlation between rises in Chlamydia pneumoniae -specific antibodies, platelet activation and lipid peroxidation after percutaneous coronary intervention European Journal of Clinical Microbiology & Infectious Diseases human EDTA plasma
2008 Ghosh et al. Platelet serotonin level and its correlation with finger length relation Indian Journal of Psychiatry human platelets
2008 Gritters et al. Platelet activation in clinical haemodialysis: LMWH as a major contributor to bio-incompatibility? Nephrology Dialysis Transplantation human K2EDTA PRP PPP
2009 Modlin er al. Principal Component Analysis, Hierarchical Clustering, and Decision Tree Assessment of Plasma mRNA and Hormone Levels as an Early Detection Strategy for Small Intestinal Neuroendocrine (Carcinoid) Tumors Ann Surg Oncol human serum
2007 Kidd et al. Further delineation of the continuous human neoplastic enterochromaffin cell line, KRJ-I, and the inhibitory effects of lanreotide and rapamycin Journal of Molecular Endocrinology human carcinoid cell line KRJ-I cell culture medium
2007 Van Buren et al. The Development and Characterization of a Human Midgut Carcinoid Cell Line Clinical Cancer Research human midgut carcinoid cell line cell lysate
2007 Soga et al. Serotonin Activates Human Monocytes and Prevents Apoptosis Journal of Investigative Dermatology human monocytes cell culture supernatant
2009 Siddique et al. KRJ-I and BON Cell Lines: Defining an Appropriate Enterochromaffin Cell Neuroendocrine Tumor Model Neuroendocrinology KRJ-I cells  
2009 Kanerva et al. Expression of Antizyme Inhibitor 2 in Mast Cells and Role of Polyamines as Selective Regulators of Serotonin Secretion PLoS one mast cell granula content
2000 Nakatani et al. Role of cytosolic phospolipase A2 in the production of lipid mediators and histamine release in mouse bone-marrow-derived mast cells Biochem J mice BMMC cell culture
2006 León-Ponte et al. Serotonin provides an accessory signal to enhance T cell activation by signaling through the 5-HT7 receptor Blood mice T-cell culture supernatant
2008 Ho-Tin-Noé et al. Platelet Granule Secretion Continuously Prevents Intratumor Hemorrhage Cancer Research mice platelet poor plasma
2009 Duerschmied et al. Serotonin stimulates platelet receptor shedding by tumor necrosis factor-alpha-converting enzyme (ADAM17) Journal of Thrombosis and Haemostasis mice PRP
2005 Song et al. Increased susceptibility to pulmonary hypertensin in heterozygous BMPR2-mutant mice Circulation mouse urine
2004 Ungerer et al. Generation of functional culture-derived platelets from CD34+ progenitor cells to study transgenes in the platelet environment Circ Res. mouse / human platelets
2005 Gilitzer et al. Retroviral infection and selection of culture-derived platelets allows study of the effect of transgenes on platelet physiology ex vivo and on thrombus…. Arterioscler mouse / human platelets
2005 Song et al. Melatonin attenuates stress-induced defecation: lesson from a rat model of stress-induced gut dysfunction Neurogastroenterol Motil rat serum
2007 Fornaro et al. Prenatal Exposure to Fluoxetine Induces Fetal Pulmonary Hypertension in the rat Am J Resp Crit Care Med rat  
2008 Papaliodis et al. The flavonoid luteolin inhibits niacin-induced flush British Journal of Pharmacology rat plasma
2009 Matondo et al. Deletion of the serotonin transporter in rats disturbs serotonin homeostasis without impairing liver regeneration American Journal of Physiology- Gastrointestinal and Liver Physiology rat blood, platelets
2009 Adams et al. Serotonergic lesions of the dorsal hippocampus differentially modulate locomotor hyperactivity induced by drugs of abuse in rats: implications for schizophrenia Psychopharmacology rat hippocampus tissue
2010 Seddik et al. Dried Leaf Extract Protects against Lead-Induced Neurotoxicity in Wistar Rats Europ J Scientific Res Wistar rats hippocampus extract
2005 Adler et al. Detection of femtogram amounts of biogenic amines using self-assembled DNA-protein nanostructures Nature Methods    
2007 Poon et al. Bystander effects of ionizing radiation can be modulated by signaling amines Enviromental Research   HPV-G cells
2007 Kopczak et al. N-nitroso-melatonin releases nitric oxide in the presence of serotonin and its derivatives Journal of Pineal Research    
2010 Svejda et al. The 5-HT2B receptor plays a key regulatory role in both neuroendocrine tumor cell proliferation and the modulation of the fibroblast component of the neoplastic microenvironment Cancer    
2010 Yu et al. Brain Perivascular Macrophages and the Sympathetic Response to Inflammation in Rats After Myocardial Infarction Hypertension    
2009 Overmyer et al. Assessment of the toxicological interaction of sertraline with cholinesterase inhibiting insecticides in aquatic insects using the black fly, Simulium vittatum IS-7 Enviromental Toxicology black fly larvae  
2007 Briffa et al. Monoamines and decision making during contests in the hermit crab Pagurus bernhardus Animal Behaviour crabs plasma
2010 Leu et al. GPCR Somatostatin Receptor Extracellular Loop 2 Is a Key Ectodomain for Making Subtype-Selective Antibodies With Agonist-Like Activities in the Pancreatic Neuroendocrine Tumor BON Cell Line Pancreas    

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