HistaSure - Dipstick

The worlds first production dip-stick for the detection of Histamine in Fish is here! In Just minutes you can screen for the presence of histamine. We pre-set the cut to international standards of 50ppm, but the system is easily changed to allow for adjustable cut-offs in order to meet your QC needs.


  1. Mix 10 grams of tuna with 240 mL of water
  2. Add 100 µl of the sample to the tube containing 500 ul of Acylation Buffer - Vortex to mix
  3. Add 100 µl of the acylation reagent to the tube in step 2
  4. Vortex to mix and incubate for 5 minutes
  5. Pipette 100 ul of the acylated sample to the Sample Diluent.
  6. Vortex to mix Add 100 ul to the cassette
  7. Read results after 5 minutes.


  • Fast results in minutes
  • No special equipment needed
  • Easy interpretation
  • Can be performed Dockside
  • Reuse the sample in our ELISA

Results and Interpretation:

The visual inspection of the LF-cassettes can give the following results: 2 lines or 1 line.

2 lines:

The histamine concentration of the sample is below 50 ppm; the sample has passed. Example: 2 lines are visible - the sample has passed


1 line:

Only the control line is visible. The histamine concentration of the sample is above 50 ppm; the sample has failed and further investigations have to be performed. Example: 1 line is visible – the sample has failed.